A PhD Position is available at the University of Exeter, Department of Biosciences, Exeter, United Kingdom
Project title: The peroxisome-ER membrane contact site (MCS) in humans
- To identify and characterise interaction partners and regulators of the novel peroxisome-ER MCS mediated by the ACBD4/5-VAPA/B tether in humans (bioinformatics, pull down assays, BioID2, mass spectrometry, molecular cell biology);
- To develop and establish models and techniques for the analysis of lipid transfer between the ER and peroxisomes;
- To validate selected candidates (CRISPR, fluorescence and quantitative electron microscopy, cell fractionation) and to reveal their physiological role in health and disease conditions;
- To examine if mutations in MCS genes can be linked to a collection of fibroblasts obtained from patients with peroxisomal disorders whose genetic bases have not yet been identified.
Location: Exeter, United Kingdom
Supervisor: Prof. Dr. M. Schrader (m.schraderATexeter.ac.uk)
Co-supervisors: Dr. M. Danielsen, MS-Omics ApS & Dr. P. Kim, The Hospital for Sick Children
Planned secondments: The Hospital for Sick Children, Academic Medical Centre at the University of Amsterdam, MS-Omics ApS
Required subject specific skills and expertise: a master’s degree in life sciences/biology (molecular cell biology, biochemistry). Experience in mammalian cell culture, fluorescence microscopy and protein biochemistry is advantageous but not mandatory.
Application deadline: 18/08/2019
Starting date: September 1, 2019
Please note: Applications that have already been submitted are well received and under consideration. No further action is required on behalf of applicants who applied before the previous deadline
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